Effects of Propamocarb Hydrochloride on Mycelial Growth, Sporulation, and Infection by Phytophthora nicotianae Isolates from Virginia Nurseries.
Identifieur interne : 001E85 ( Main/Exploration ); précédent : 001E84; suivant : 001E86Effects of Propamocarb Hydrochloride on Mycelial Growth, Sporulation, and Infection by Phytophthora nicotianae Isolates from Virginia Nurseries.
Auteurs : Jiahuai Hu ; Chuanxue Hong ; Erik L. Stromberg ; Gary W. Moorman [États-Unis]Source :
- Plant disease [ 0191-2917 ] ; 2007.
Abstract
Propamocarb hydrochloride is a systemic fungicide commonly used for control of Phytophthora diseases of nursery crops. Here we report on the effect of this compound on different growth stages of Phytophthora nicotianae, a major pathogen of numerous herbaceous and some woody ornamental plants. A total of 71 isolates were assayed for sensitivity to propamocarb at two concentrations of 1.8 mg/ml (label rate) and 10 mg/ml using clarified V8 agar as a base medium. All isolates grew at 10 mg/ml with the most sensitive isolate having 34.8% relative growth compared with growth on nonamended medium. Nine isolates were selected and further tested for mycelial growth at 0, 1, 10, 25, 50, and 100 mg/ml, and for sporangium production, zoospore motility, and germination at 0, 5, 50, 500, 5,000, and 50,000 μg/ml. EC50 values ranged from 2.2 to 90.1 mg/ml for mycelial growth, 133.8 to 481.3 μg/ml for sporangium production, 88.1 to 249.8 μg/ml for zoospore motility, and 1.9 to 184.6 μg/ml for zoospore germination, respectively. In addition, 17 selected isolates were evaluated for propamocarb sensitivity on Pelargonium × hortorum cv. White Orbit. Two days after seedlings were treated with propamocarb at 3.6 mg/ml, they were inoculated by either inverting one 5-mm-diameter plug of a 3-day-old culture or applying a 10-μl drop containing 20 zoospores onto each cotyledon. Propamocarb hydrochloride provided good protection of geranium seedlings from zoospore infections but not from mycelial infections. These results suggest that this fungicide must be used preventively for Phytophthora disease management and that mycelial growth may not be the most reliable measurement to determine the development of fungicide resistance to this compound in Phytophthora species at production facilities and in the landscape.
DOI: 10.1094/PDIS-91-4-0414
PubMed: 30781183
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Effects of Propamocarb Hydrochloride on Mycelial Growth, Sporulation, and Infection by Phytophthora nicotianae Isolates from Virginia Nurseries.</title><author><name sortKey="Hu, Jiahuai" sort="Hu, Jiahuai" uniqKey="Hu J" first="Jiahuai" last="Hu">Jiahuai Hu</name><affiliation><nlm:affiliation>Department of Plant Pathology, Physiology and Weed Science, Virginia Polytechnic Institute and State University, Hampton Roads Agricultural Research and Extension Center, Virginia Beach 23455.</nlm:affiliation><wicri:noCountry code="subField">Virginia Beach 23455</wicri:noCountry></affiliation></author><author><name sortKey="Hong, Chuanxue" sort="Hong, Chuanxue" uniqKey="Hong C" first="Chuanxue" last="Hong">Chuanxue Hong</name><affiliation><nlm:affiliation>Department of Plant Pathology, Physiology and Weed Science, Virginia Polytechnic Institute and State University, Hampton Roads Agricultural Research and Extension Center, Virginia Beach 23455.</nlm:affiliation><wicri:noCountry code="subField">Virginia Beach 23455</wicri:noCountry></affiliation></author><author><name sortKey="Stromberg, Erik L" sort="Stromberg, Erik L" uniqKey="Stromberg E" first="Erik L" last="Stromberg">Erik L. Stromberg</name><affiliation><nlm:affiliation>Department of Plant Pathology, Physiology and Weed Science, Virginia Polytechnic Institute and State University, Blacksburg 24061.</nlm:affiliation><wicri:noCountry code="subField">Blacksburg 24061</wicri:noCountry></affiliation></author><author><name sortKey="Moorman, Gary W" sort="Moorman, Gary W" uniqKey="Moorman G" first="Gary W" last="Moorman">Gary W. Moorman</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant Pathology, The Pennsylvania State University, University Park 16802.</nlm:affiliation><orgName type="university">Université d'État de Pennsylvanie</orgName><country>États-Unis</country><placeName><settlement type="city">University Park (Pennsylvanie)</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2007">2007</date><idno type="RBID">pubmed:30781183</idno><idno type="pmid">30781183</idno><idno type="doi">10.1094/PDIS-91-4-0414</idno><idno type="wicri:Area/Main/Corpus">001F88</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">001F88</idno><idno type="wicri:Area/Main/Curation">001F88</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">001F88</idno><idno type="wicri:Area/Main/Exploration">001F88</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Effects of Propamocarb Hydrochloride on Mycelial Growth, Sporulation, and Infection by Phytophthora nicotianae Isolates from Virginia Nurseries.</title><author><name sortKey="Hu, Jiahuai" sort="Hu, Jiahuai" uniqKey="Hu J" first="Jiahuai" last="Hu">Jiahuai Hu</name><affiliation><nlm:affiliation>Department of Plant Pathology, Physiology and Weed Science, Virginia Polytechnic Institute and State University, Hampton Roads Agricultural Research and Extension Center, Virginia Beach 23455.</nlm:affiliation><wicri:noCountry code="subField">Virginia Beach 23455</wicri:noCountry></affiliation></author><author><name sortKey="Hong, Chuanxue" sort="Hong, Chuanxue" uniqKey="Hong C" first="Chuanxue" last="Hong">Chuanxue Hong</name><affiliation><nlm:affiliation>Department of Plant Pathology, Physiology and Weed Science, Virginia Polytechnic Institute and State University, Hampton Roads Agricultural Research and Extension Center, Virginia Beach 23455.</nlm:affiliation><wicri:noCountry code="subField">Virginia Beach 23455</wicri:noCountry></affiliation></author><author><name sortKey="Stromberg, Erik L" sort="Stromberg, Erik L" uniqKey="Stromberg E" first="Erik L" last="Stromberg">Erik L. Stromberg</name><affiliation><nlm:affiliation>Department of Plant Pathology, Physiology and Weed Science, Virginia Polytechnic Institute and State University, Blacksburg 24061.</nlm:affiliation><wicri:noCountry code="subField">Blacksburg 24061</wicri:noCountry></affiliation></author><author><name sortKey="Moorman, Gary W" sort="Moorman, Gary W" uniqKey="Moorman G" first="Gary W" last="Moorman">Gary W. Moorman</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant Pathology, The Pennsylvania State University, University Park 16802.</nlm:affiliation><orgName type="university">Université d'État de Pennsylvanie</orgName><country>États-Unis</country><placeName><settlement type="city">University Park (Pennsylvanie)</settlement><region type="state">Pennsylvanie</region></placeName></affiliation></author></analytic><series><title level="j">Plant disease</title><idno type="ISSN">0191-2917</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Propamocarb hydrochloride is a systemic fungicide commonly used for control of Phytophthora diseases of nursery crops. Here we report on the effect of this compound on different growth stages of Phytophthora nicotianae, a major pathogen of numerous herbaceous and some woody ornamental plants. A total of 71 isolates were assayed for sensitivity to propamocarb at two concentrations of 1.8 mg/ml (label rate) and 10 mg/ml using clarified V8 agar as a base medium. All isolates grew at 10 mg/ml with the most sensitive isolate having 34.8% relative growth compared with growth on nonamended medium. Nine isolates were selected and further tested for mycelial growth at 0, 1, 10, 25, 50, and 100 mg/ml, and for sporangium production, zoospore motility, and germination at 0, 5, 50, 500, 5,000, and 50,000 μg/ml. EC<sub>50</sub> values ranged from 2.2 to 90.1 mg/ml for mycelial growth, 133.8 to 481.3 μg/ml for sporangium production, 88.1 to 249.8 μg/ml for zoospore motility, and 1.9 to 184.6 μg/ml for zoospore germination, respectively. In addition, 17 selected isolates were evaluated for propamocarb sensitivity on Pelargonium × hortorum cv. White Orbit. Two days after seedlings were treated with propamocarb at 3.6 mg/ml, they were inoculated by either inverting one 5-mm-diameter plug of a 3-day-old culture or applying a 10-μl drop containing 20 zoospores onto each cotyledon. Propamocarb hydrochloride provided good protection of geranium seedlings from zoospore infections but not from mycelial infections. These results suggest that this fungicide must be used preventively for Phytophthora disease management and that mycelial growth may not be the most reliable measurement to determine the development of fungicide resistance to this compound in Phytophthora species at production facilities and in the landscape.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30781183</PMID><DateRevised><Year>2019</Year><Month>11</Month><Day>20</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0191-2917</ISSN><JournalIssue CitedMedium="Print"><Volume>91</Volume><Issue>4</Issue><PubDate><Year>2007</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Plant disease</Title><ISOAbbreviation>Plant Dis</ISOAbbreviation></Journal><ArticleTitle>Effects of Propamocarb Hydrochloride on Mycelial Growth, Sporulation, and Infection by Phytophthora nicotianae Isolates from Virginia Nurseries.</ArticleTitle><Pagination><MedlinePgn>414-420</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/PDIS-91-4-0414</ELocationID><Abstract><AbstractText>Propamocarb hydrochloride is a systemic fungicide commonly used for control of Phytophthora diseases of nursery crops. Here we report on the effect of this compound on different growth stages of Phytophthora nicotianae, a major pathogen of numerous herbaceous and some woody ornamental plants. A total of 71 isolates were assayed for sensitivity to propamocarb at two concentrations of 1.8 mg/ml (label rate) and 10 mg/ml using clarified V8 agar as a base medium. All isolates grew at 10 mg/ml with the most sensitive isolate having 34.8% relative growth compared with growth on nonamended medium. Nine isolates were selected and further tested for mycelial growth at 0, 1, 10, 25, 50, and 100 mg/ml, and for sporangium production, zoospore motility, and germination at 0, 5, 50, 500, 5,000, and 50,000 μg/ml. EC<sub>50</sub> values ranged from 2.2 to 90.1 mg/ml for mycelial growth, 133.8 to 481.3 μg/ml for sporangium production, 88.1 to 249.8 μg/ml for zoospore motility, and 1.9 to 184.6 μg/ml for zoospore germination, respectively. In addition, 17 selected isolates were evaluated for propamocarb sensitivity on Pelargonium × hortorum cv. White Orbit. Two days after seedlings were treated with propamocarb at 3.6 mg/ml, they were inoculated by either inverting one 5-mm-diameter plug of a 3-day-old culture or applying a 10-μl drop containing 20 zoospores onto each cotyledon. Propamocarb hydrochloride provided good protection of geranium seedlings from zoospore infections but not from mycelial infections. These results suggest that this fungicide must be used preventively for Phytophthora disease management and that mycelial growth may not be the most reliable measurement to determine the development of fungicide resistance to this compound in Phytophthora species at production facilities and in the landscape.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Jiahuai</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Physiology and Weed Science, Virginia Polytechnic Institute and State University, Hampton Roads Agricultural Research and Extension Center, Virginia Beach 23455.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hong</LastName><ForeName>Chuanxue</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Physiology and Weed Science, Virginia Polytechnic Institute and State University, Hampton Roads Agricultural Research and Extension Center, Virginia Beach 23455.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Stromberg</LastName><ForeName>Erik L</ForeName><Initials>EL</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, Physiology and Weed Science, Virginia Polytechnic Institute and State University, Blacksburg 24061.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Moorman</LastName><ForeName>Gary W</ForeName><Initials>GW</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, The Pennsylvania State University, University Park 16802.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Dis</MedlineTA><NlmUniqueID>9882809</NlmUniqueID><ISSNLinking>0191-2917</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">IPM</Keyword><Keyword MajorTopicYN="N">fungicide resistance</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>2</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>4</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>4</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30781183</ArticleId><ArticleId IdType="doi">10.1094/PDIS-91-4-0414</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Pennsylvanie</li></region><settlement><li>University Park (Pennsylvanie)</li></settlement><orgName><li>Université d'État de Pennsylvanie</li></orgName></list><tree><noCountry><name sortKey="Hong, Chuanxue" sort="Hong, Chuanxue" uniqKey="Hong C" first="Chuanxue" last="Hong">Chuanxue Hong</name><name sortKey="Hu, Jiahuai" sort="Hu, Jiahuai" uniqKey="Hu J" first="Jiahuai" last="Hu">Jiahuai Hu</name><name sortKey="Stromberg, Erik L" sort="Stromberg, Erik L" uniqKey="Stromberg E" first="Erik L" last="Stromberg">Erik L. Stromberg</name></noCountry><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Moorman, Gary W" sort="Moorman, Gary W" uniqKey="Moorman G" first="Gary W" last="Moorman">Gary W. Moorman</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhytophthoraV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001E85 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001E85 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhytophthoraV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:30781183
|texte= Effects of Propamocarb Hydrochloride on Mycelial Growth, Sporulation, and Infection by Phytophthora nicotianae Isolates from Virginia Nurseries.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:30781183" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhytophthoraV1
| This area was generated with Dilib version V0.6.38. |  |